Fluorescent pH-responsive polymer for cell imaging and cellular pH monitoring

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Important Dates

Conference:

Apr. 22-24, 2019

Full Paper Due: Mar. 18, 2019

Abstract Due: Mar. 18, 2019

Audience Registration Due:
Apr. 22, 2019

Presentations of The Spring World Congress on Engineering and Technology (SCET 2017)

● Fluorescent pH-responsive polymer for cell imaging and cellular pH monitoring
Author(s)
Zhaoyang Ding
Affiliation(s)
University of Macau
KEYWORDS
Fluorescent pH-responsive polymer, cell imaging, cellular pH monitoring
ABSTRACT
Both intracellular and extracellular pH values play significant roles on cell metabolism and growth. Due to the precise imaging capabilities, fluorescent probes have attracted great attention for the illustration of pH modulated processes. Detecting cellular pH values with high throughput is critical for cell research and applications. In this research, a series of fluorescent pH-responsive polymers were developed and characterized for cell imaging and exclusive detection of cellular pH values. Poly(dimethylaminoethyl methacrylate) is used as the host polymer to increase the water solubility and pH responsive ability of the polymers without introducing cell toxicity. The modified fluorescein was used as the fluorescent part of the polymers, and emission spectra from the polymeric sensor under excitation at the isosbestic point 450 nm possess a fluorescence peak at 525 nm. One of the polymer could detected the intercellular pH values. Results showed that the pH sensor can be used for detection of pH values with high repeatability and stability.